By Naren Bhokisham

The article below, from the journal Bioengineering, concludes, “similar to how DNA based vaccines are being electroporated into the human body to fight Covid-19, we may expect that electrogenetic promoter circuits will be inserted into human cells to open a new modality of bioelectronic signaling.”

As Patrick Wood of Technocracy.News points out, the ultimate hacking of the human body will be “electrogenetics,” where human DNA can be selectively switched on and off by electronic signals.

Just like Internet of Things (IoT), the Internet of Bodies (IoB) refers to access and control of the human body via the internet. Here, we detail how CRISPR can be used to electrically connect with the genome and as a proof of concept display control over transcriptional informational networks inside E. coli and Salmonella.

Technology has played a transformative role in our lives and its impact on human health is never felt more than in the current times of the Covid-19 global pandemic.

In this scenario, development of autonomous health sensing and actuating systems, also referred to as closed loop systems that ‘sense’ and ‘act’ towards a biological condition (Kovatchev et al., 2009; Berényi et al., 2012), can play a critical role in addressing health crises of the future. Successful adoption of electronic closed loop human health systems is dependent on the development of new methods for biological actuation which has so far been limited to the century old neural stimulation and optogenetics.

Recent advances in the field of biological actuation stems from synthetic biology where our group and others have reported gene circuits that respond to electric signals with expression of specific gene of interests (Weber et al., 2008; Tschirhart et al., 2017; Krawczyk et al., 2020). In a prior publication, our group had detailed a redox-based bacterial promoter SoxS that responds to specific electrochemical signals that can be generated via an external electrode.

Using this promoter, specific transgenes of interests can be expressed in bacteria in response to programmed electric stimuli. In this work, we have taken the next logical leap for this technology, that is, using electric signals to connect and control transcriptional networks in the genome of the cells (Bhokisham et al., 2020).

To aid in our pursuit, we exploited the CRISPR technology that provides the means to target any specific target in the genome. Specifically, we used the dCas9 based transcriptional activator to electrically activate and repress select genes of interests.

Firstly, we integrated the CRISPR system with the SoxR based electro responsive promoter, optimized various components involved in the CRISPR system to make a tunable and inducible system. In this way, using CRISPR we electrically activated LasI, an autoinducer-1 (AI-1) synthase resulting in generation of AI-1, a quorum sensing mediator (Fig. 2 and 3).

Later, we repurposed the CRISPR activator to simultaneously repress select genes as well. Since the electrochemical stimuli that drives the  SoxS promoter also induces oxidative stress, bacterial cells activate intrinsic stress defense responses to attenuate the electrical stimuli. We used the repurposed CRISPR activator to repress the activation of oxidative stress defenses in E. coli and S. enterica, thus leading to enhanced output from the electrical stimuli responsive promoter (Fig 4).

When placed in the context of the complex spatio-temporal signal gradients at the bioelectronic interface, cells with repressed oxidative stress defenses displayed more aligned responses in relation to the external signal gradients (Fig. 5).

This concept of silencing certain elements in the genome in order to maintain better congruence with external environments is nature inspired and found in embryogenesis and yeast as well (Yu et al., 2008; Paulsen et al.,2011).

Read the entire story at Bioengineering.

by Leo Hohmann

A petition drive may have helped save the lives of two Georgia residents who were trapped in a hospital that refused to respect their daughter’s power of attorney and release them to another medical facility that would administer critical life-saving treatments.

Joseph and Beverly Rickels were both admitted to Piedmont Hospital in Cartersville, Georgia, on Sunday, Sept. 12, diagnosed with Covid-related pneumonia. The condition of both husband and wife has now deteriorated to the point where both are receiving oxygen. Joseph is in critical condition.

LifeSite News, a conservative media organization, asked people Thursday to sign a petition requesting the governor to step in and allow the daughter to get her parents out of Piedmont Hospital and into another facility that will give the treatments they need.

Shockingly, the attending physician said that she would not release the patients to their daughter’s care unless she (the daughter) proves she is vaccinated.

See our previous article explaining how hospitals nationwide are complying with top-down CDC guidelines focused on a vaccine-exclusive approach that is actually killing people.

Word came late Friday afternoon, Sept. 17, that the petition campaign was yielding results. Officials at Piedmont Hospital had finally conceded to let the couple see any doctor of their choice who could come to the hospital and give them the treatment they requested, but that decision came days late and only after extensive public pressure had been applied on the hospital, Steve Jalsevec, president and co-founder of LifeSite News, told LeoHohmann.com.

“Crucial time was lost in dealing with their obstinance and denying the use of critical medications that are known to work all around the world, and then they wouldn’t release the patients to their daughter who had power of attorney, simply because she was unvaccinated,” Jalsevec said.

The LifeSite email alert sent out Thursday asked readers to “urgently CONTACT Governor Brian Kemp to intervene in this case and demand that Piedmont Hospital respect the power of attorney of their daughter, and the desire of Joseph and Beverly Rickels to be moved to another location to receive the care of their choice.”

The couple’s daughter, Courtney Rimmer, has power of attorney, which should, by law, allow her to make decisions for her parents, both of whom indicated they agree to the move.

You can still call or email the Governor’s Office to let him know your displeasure on how this hospital is failing its patients. The number is (404) 656-1776.

The Rickels have the right to receive fully-approved, known safe alternative treatments such as Ivermectin, and this hospital did not respect their duly-appointed power of attorney, as well as medical privacy, until it was possibly too late.

If Piedmont is denying critical treatment to the Rickels, they are likely doing the same to other Covid patients. This must stop.

It might also be a good idea to contact Georgia State Attorney General Chris Carr to ask that his office investigate the medical tyranny that is overtaking Georgia’s hospitals. The Attorney General’s number is: (404) 458-3600.

If this can happen to the Rickels, it can happen to anyone.

Piedmont Healthcare is a private nonprofit operating 16 hospitals and hundreds of clinics throughout Georgia.

The hospital chain claims on its website factsheet to “provide safe, compassionate, convenient, and high-quality care across 16 hospitals, 50 Piedmont Urgent Care centers, 25 QuickCare locations, 610 Piedmont Clinic physician practice locations and more than 2,770 Piedmont Clinic members.”

If Piedmont is truly interested in “high-quality, compassionate care,” it would not have delayed the administering of critical treatments to the Rickels. It would not need to receive dozens of calls and/or emails to remind them how to make such basic good-faith decisions. And it certainly would not be asking for the vaccine status of a worried daughter before honoring her wishes as power of attorney for her parents.

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